Unlike cDNA arrays, This may not be as simple with Affi arrays. However, it is possible to analyze the results for highly conserved genes like actin provided...
Hi, Can anyone help me in analyzing PCAG data. What are the values that are plotted in the PCAG?Is it based on eigen values or based on the vector values of...
Dear Microarray Members: The venture community is starting to look again at microarray teams. Please join our forum and find out who is getting funded and...
Hi all, I d like to know if it is necessary or recommended to filter the data according to the Mean / SD ratio for replicates before running a comparison...
phguardiol@...
Jul 1, 2003 8:13 pm
10304
We are offering a rerun of our hands-on workshops in Boston, at UMASS Boston. The dates this time will be July 29-31, 2003 The Quantitative PCR workshop runs...
Duncan Talbot
talbot3@...
Jul 2, 2003 3:04 am
10305
Dear Colleagues, Currently we need to convert the .chp file (chip file) to excel format using Affymetrix software. The biologist need to select manually the...
Lariani,Sofiane,LAUSA...
GENE-ARRAYS@...
Jul 2, 2003 3:27 am
10306
Hi all I am wondering if in this case : two colors hybridization on glass slide for microarray analysis. the cy3 is the RNA reference and cy5 is the RNA from...
A Question to All: We have a hypothesis that gene fusion happens in cancer cell much earlier than gene amplification, especially in colon cancer. Is there a...
Now at http://rfinney.com/cel2gif.html Source Code / Binaries / Windows Support / Affymetrix CEL and DAT files supported. I now have binaries for Windows and...
Dear Members: There is tremendous interest in the Tier 1 Venture community with genechip arrays for DNA/mRNA analysis/genechip expression arrays with other...
I will be out of the office starting 30/06/2003 and will not return until 14/07/2003. I will be out of the office form 1st July 2003, returning on 14th July...
Marcus Wills
MWills@...
Jul 3, 2003 12:05 am
10311
Sofiane, The .CHP file can be exported as a .txt file, then just write a macro in excel to remove the columns you do not want and perform any formatting that ...
John F Schwedes
jschwedes@...
Jul 3, 2003 3:11 am
10312
Hi, I don't have a solution to your problem (I have the same one), but here are the procedures I use when working in MAS to extract MAS-derived metrics from ...
Eric Blalock
GENE-ARRAYS@...
Jul 3, 2003 3:22 am
10313
Sofiane, My best suggestion would be to use the Affymetrix Data Mining Tool, which should be installed as part of the Affymetrix Suite on your system provided...
Jeff Potter
GENE-ARRAYS@...
Jul 3, 2003 3:23 am
10314
I wanted to briefly share my experience with Spotfire Decision Site for Functional Genomics. I am still a beginner in the field of genomics, and my learning...
James Luyendyk
luyendyk@...
Jul 3, 2003 3:28 am
10315
You could take a look at this, they can incorporate batches in their analysis: http://www.esat.kuleuven.ac.be/maran/index.jsp Greetings...
Kheros_11
kheros_11@...
Jul 3, 2003 8:56 am
10316
Hi we are planning to immobilize cells using agarose gel. In order to do this, the gel should have a very low melting point, 30-37¡æ, while the lowest...
Dear microarray users, Recently we have been facing a problem scanning our microarray images and would like to know if anyone has run into the same kind of ...
STRICTLY CONFIDENTIAL DR. JOSEPH ARIDA FEDERAL SECRETARIAL COMPLEX FALOMO, LAGOS-NIGERIA EMAIL ADDRESS josepharida@.... FOR YOUR KIND ATTENTION, WE...
JOSEPH ARIDA
josepharida@...
Jul 3, 2003 7:58 pm
10319
STRICTLY CONFIDENTIAL DR. JOSEPH ARIDA FEDERAL SECRETARIAL COMPLEX FALOMO, LAGOS-NIGERIA EMAIL ADDRESS josepharida@.... FOR YOUR KIND ATTENTION, WE...
JOSEPH ARIDA
josepharida@...
Jul 3, 2003 7:59 pm
10320
Consider Matrigel instead of agarose. It is tissue culture compatible and the matrix is liquid at 2-8C and gels rapidly at 22C to 37C. The gel is protein based...
Hello, I usually export data to excel from DMT (not MAS) using "pivot data" function and then select all => copy => paste. DMT remembers the settings of pivot...
Oleg V. Moskvin
ovm@...
Jul 4, 2003 3:09 am
10322
Do anyone have a comprehensive list of housekeeping genes for E.Coli? Thanks. Anyou Wang 322 Science Lab I Uni. of CA, Riverisde ...
ANYOU WANG
GENE-ARRAYS@...
Jul 4, 2003 3:10 am
10323
Dear all, does anyone know the "real" number of genes represented on the U95A chip from affymetrix. I found that there are quite a number of duplicates and ...
Claus Reimertz
GENE-ARRAYS@...
Jul 4, 2003 3:27 am
10324
Please see the attached zip file for details. [Non-text portions of this message have been removed]...
alodiaz@...
Jul 4, 2003 7:40 am
10325
Just be aware that matrigel is not an inert substrate. It has many known biological activities that could affect your cell's behavior. A simpler substrate may...
try this site http://www.ngelab.org/workshop.htm <http://www.ngelab.org/workshop.htm> . Kathryn Payne Institute of Neuroscience Carleton University Ottawa...
Kathryn_Payne
GENE-ARRAYS@...
Jul 5, 2003 3:20 am
10327
Claus, There are 8103 distinct UniGene clusters (11258 accession numbers) represented on the HG95Av2. If you have a hypothesis about the phenomenon you are...
Sorin Draghici
GENE-ARRAYS@...
Jul 5, 2003 3:29 am
10328
Dear all, I have performed GeneChip experiments (U95A) with an ER stress stimulus which is known to activate a transcriptional response. The analysis revealed,...