Nick Tinker is out of the office until August 11. If this is urgent, please contact one of the following: Hai Pham (phamh@...) Charlene Wight (Wightcp@....) ...
Nick Tinker
tinkerna@...
Aug 1, 2003 11:45 am
10610
I recall a previous discussion on here in which the consensus was that small fold changes can be important. It is quite common to find very small changes to...
Good afternoon, I'd like to seek some advice: we have a new Affymetrix system. We are planning to run some arrays for precious RNA samples purified with...
... Thanks for thr reply, but is it a software that I can use to do that for me? or if its embede in any analysis software. regards ... for each expression...
... How did you do your washes? I normally wash three times using 700ul washing buffer and never had a background problem. ... I've tried to use 1/4 of the...
Hao Chen
hchen@...
Aug 2, 2003 2:04 am
10616
Bono, You should be aware of the fact that the method of selecting differentially regulated genes by using a fold change cut-off has several important flaws....
Sorin Draghici
GENE-ARRAYS@...
Aug 2, 2003 2:15 am
10617
Hello all! There two virtual seminars on oligo purification (can be for microarrays and siRNA studies) in August. Go to http://transgenomicevents.webex.com and...
Alexander Kuklin
kuklina@...
Aug 2, 2003 2:15 am
10618
Dear Stephanie, The Qiagen Qiaquick PCR purification kit works extremely well for the two-step (indirect) labeling approach for two reasons. First, ...
Greg Cox
GENE-ARRAYS@...
Aug 2, 2003 2:16 am
10619
A PhD and a postdoc position (three years funding) are available at Marburg University, Germany, in the Institute of Physiology. We analyze functional and...
B.Liss Oxford
birgit.liss@...
Aug 2, 2003 2:16 am
10620
Hi, just a comment about the recent questions of finding one "magic" and universal fold change cut off: It does not exist! As the noise in the *observed*...
Henrik Bengtsson
hb@...
Aug 2, 2003 2:17 am
10621
Hello Andreas, NuGEN recently introduced a new linear amplification technology which produces micrograms of cDNA from as little as 5 ng total RNA in less than...
Ellen Soo Hoo
GENE-ARRAYS@...
Aug 2, 2003 2:20 am
10622
Bono: Numerous experts on analysis have reported that fold-change is a 'disaster' with respect to sensitivity (ability to detect genes that are truly ...
Lyons-Weiler, James
GENE-ARRAYS@...
Aug 2, 2003 2:21 am
10623
... YES!! DO NOT USE FOLD CHANGE!! Use a real statistical model with actual statistical properties! -- David A. Henderson, Ph.D. Assistant Professor of...
David Henderson
GENE-ARRAYS@...
Aug 2, 2003 2:22 am
10624
Hello Mirva, i have also been using amershams cyscribe cDNA labeling kit for the past 6 months.I am also facing the same problems which you have mentioned...
Dear List Members, NimbleGen Systems, a high-density oligo array manufacturer, has an immediate opening for a Technical Sales Representative to join our Sales...
Emile Nuwaysir
GENE-ARRAYS@...
Aug 2, 2003 2:23 am
10626
Nick Tinker is out of the office until August 11. If this is urgent, please contact one of the following: Hai Pham (phamh@...) Charlene Wight (Wightcp@....) ...
Nick Tinker
tinkerna@...
Aug 2, 2003 2:24 am
10627
Hello, I'm searching for an easy-to-use microarray analysis tool. My experimental condition is very simple. There's only one time point, so any clustering...
Nick Tinker is out of the office until August 11. If this is urgent, please contact one of the following: Hai Pham (phamh@...) Charlene Wight (Wightcp@....) ...
Nick Tinker
tinkerna@...
Aug 2, 2003 10:24 am
10629
It's a topic set by my supervisor in the university (first year). I have been researching a little and still can't find a solution due to the some problems...
skytsai
skytsai@...
Aug 4, 2003 7:31 am
10630
Does anyone have any experience of using reflective metalised slides such as those from Erie? They claim to give increases in signal-noise ratio. I was...
Dear Patty, Erie Scientific manufactures affordable microarray substrates for long oligo arrays. The SuperChip microarray slides are available through Fisher...
Hi Folks I should have double checked. We actually use 150 mM NaPO4 pH 8.5, 0.001% SDS and not 0.01% SDS as I said. The higher SDS concentration works OK but...
Bart Janssen
GENE-ARRAYS@...
Aug 5, 2003 2:11 am
10633
Thank you for your recent email. I have a new email address that you should send to. It is lee01@.... Please make a note of this for all future...
David S. Lee
david.lee@...
Aug 5, 2003 2:11 am
10634
Thank you for your recent email. I have a new email address that you should send to. It is lee01@.... Please make a note of this for all future...
David S. Lee
david.lee@...
Aug 5, 2003 2:20 am
10635
hi 2. i had good qpcr reproducibility using trizol RNA/superscriptII cDNA from patient samples. see ref: seth et al 2003. Journal of Biochemical & Biophysical...
Devanshi Seth
GENE-ARRAYS@...
Aug 5, 2003 2:20 am
10636
Thank you for your recent email. I have a new email address that you should send to. It is lee01@.... Please make a note of this for all future...
David S. Lee
david.lee@...
Aug 5, 2003 2:20 am
10637
Please see "Identifying differentially expressed genes from microarray data with the Limit fold Change (LFC) Model" by mucth et al. in ...
Alexander Kuklin
kuklina@...
Aug 5, 2003 2:21 am
10638
Thank you for your recent email. I have a new email address that you should send to. It is lee01@.... Please make a note of this for all future...
