Hello Mandy, I recommend the labeling protocol that we have developed for our microarray users: http://microarray.vai.org/protocols/ We have very reliable,...
Hello all, Our lab recently purchased a Perkin Elmer Scanarray Express HT microarray scanner. The new feature we were most looking forward to was a 20-slide...
FROM: DR ADEBAYO SMITH REQUEST FOR URGENT BUSINESS RELATIONSHIP FIRST, I MUST SOLICIT YOUR CONFIDENCE IN THIS TRANSACTION. THIS IS VIRTURE OF IT'S NATURE AS...
DR. ADEBAYO SMITH
adebayo_smith@...
Oct 1, 2003 4:16 pm
11452
Pete, Is the problem caused by photobleaching, or is it possible it is poor adhesion of the dots over an extended time period. I am not used to your industry...
Hi all, I have been using the Roche cDNA synthesis kit combined with Ambion T7 megascript kit to prepare amplified cRNA from total RNA. Lately I have noticed...
My boyfriend cheated on me last weekend and I am looking for a little payback. I am a SWF, 24, 118 PDS, brunette, blue eyes and a ass for days. If you want to...
Dear all This may seem a silly question, but I want to know how to calculate correlation between multiple replicate microarray experiments. If I do 5 parallel...
Gerard
vesarat.wessagowit@...
Oct 2, 2003 2:05 am
11457
Sam, One possible reason: We had a bad lot of BSA that gave us a high background signal, about 5 to 6 times higher than normal. Changing the lot of the BSA ...
Jeanette McClintick
jnmcclin@...
Oct 2, 2003 2:10 am
11458
Hi Cathy, I found blocking to be effective even after three weeks time in the storage conditions that you mentioned. Iram Iram Rishi, M.D. Manager Microarray...
Iram Rishi
irishi@...
Oct 2, 2003 2:15 am
11459
Dear All, We are interested in buying a used affy scanner. Please feel free to contact me directly if you have one. Many thanks! Kind Regards, Youxiang Wang,...
GENE-ARRAYS@...
Oct 2, 2003 2:17 am
11460
Hi Samuel Yes, this high background is a common problem with affy chips. We have had it and know of others who have also experienced it. Contact Affy and you ...
It would seem logical to calculate the correlation coefficient if you have 2 replicates, and I'm guessing that you can use the Student's t distribution, with...
hi all we are considering buying a scanner from the LS series (200/300) I would like to get some comments about it from users who had experience with it...
We also purchased a Scanarray HT scanner recently and are also having the same problems with photobleaching as Peter. We have run experiments to test this...
Sonya Kamdar
sjk2@...
Oct 2, 2003 1:57 pm
11467
Hi, I'm looking for information on the hybridization efficiency of both spotted and affy chips. I've heard that in a typical expression analysis experiment,...
truro212
truro212@...
Oct 2, 2003 2:33 pm
11468
A quick question, I would like to know how people are isolating total RNA and what is the best small scale protocol for the affymetrix system. I'm isolating...
What you are looking for is the intraclass correlation coefficient which is estimated using estimates of variance components, specifically the variance between...
Photobleaching is due to atmospheric ozone and not your scanner. See "Effects of Atmospheric Ozone on Microarray Data Quality" Thomas L. Fare, Ernest M....
rbchadwick
rbchadwick@...
Oct 2, 2003 5:20 pm
11471
RB, As a microarray core facility, our lab is quite familiar with the paper that you reference. We still have good reason to believe that atmospheric ozone is...
Actually I think my previous post was for a different model, here is an alternate intraclass correlation which fits the microarray problem better: The error...
BITTE BEACHTEN SIE UNSERE NEUE E-MAIL-ADRESSE ! Die neue E-Mail Adresse setzt sich zusammen aus Vorname.Nachname@... z.B. Thomas.Mustermann@... Mit...
notification@...
Oct 2, 2003 8:25 pm
11474
One of the controlled experiments we set up was to compare the Scanarray HT with our Axon Scanner. The results showed that the Axon scanned slides did not...
Sonya Kamdar
sjk2@...
Oct 2, 2003 9:58 pm
11475
Dear Group members We are starting again the monthly Virtual Seminars on Genomics and Bioinformatics. The next virtual seminar will be presented by Dr. Irene...
