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Filtros FM. Milenia
Filtros@...
Feb 1, 2004 9:38 am
13025
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brenda@...
Feb 1, 2004 1:36 pm
13026
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Chadwick Dodd
aohgnu@...
Feb 1, 2004 2:15 pm
13027
If you enjoy masturbation maybe you should try sex with a partner. Find yourself a hot slut to poke at this spot! ...
Hi, everybody, I am trying to measure how many oligonucleotide molecules (# molecules/um2) I have immobilized on glass slide surface. Can anybody recommend a...
Hi all, I am trying to perform hierarchical clustering on some 15,000 genes using Axon's software. I have 1GB of RAM, but my PC keeps reading "out of memory"...
The message cannot be represented in 7-bit ASCII encoding and has been sent as a binary attachment. [Non-text portions of this message have been removed]...
okker@...
Feb 2, 2004 4:28 pm
13034
Hi Sabz, You are running out of memory. If you cluster 15000+ genes, the distance matrix has 15000 x 15000 /2 elements. Since stable genes are not informative,...
i sure someone prints mouse cpg island arrays, can ayone remind me who?...
dstorton
dstorton@...
Feb 2, 2004 9:42 pm
13038
Hello, I am new to microarray work. Recently I am facing problem in my reverse transcription reaction (I am using bacterial RNA and qiagen column isoaltion)....
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Carlos Aguirre
vqplhwjr@...
Feb 2, 2004 11:03 pm
13040
Dear all, I am using RNA from rat brain in my microarray experiments. What is the best reference RNA to use my experiments? Thanks Cleo ...
Dr Cleo Hughes
GENE-ARRAYS@...
Feb 3, 2004 4:02 am
13041
We have used my Biomek FX to cherrypick plasmids for PCR setup and to set up the PCR reactions (including template dilution) in 96-well plates. We also used it...
STKH (Steen Krogsgaard)
GENE-ARRAYS@...
Feb 3, 2004 4:09 am
13042
I did some reading up on RNA storage and that brought up a question. Ambion's website suggests that for long-term storage, keep the RNA as an ethanol...
Jennifer Huff
jlmshuff@...
Feb 3, 2004 4:16 am
13043
David, There are couple of web resources that make microarray data available. you might try http://lgsun.grc.nia.nih.gov/microarray/data.html or data from...
Lesley C Taylor
GENE-ARRAYS@...
Feb 3, 2004 4:17 am
13044
Dear Remo, not knowing, if your request is still actual, please check on www.memorec.de for information about our array configurations. We offer a skin...
Barbara Schaffrath
GENE-ARRAYS@...
Feb 3, 2004 4:18 am
13045
Here is a question we were recently asked: As Affymetrix GeneChips are measuring single nucleotide changes for mismatch: Does it matter what strain of mice is...
Mark Band
GENE-ARRAYS@...
Feb 3, 2004 4:23 am
13046
POSTDOCTORAL FELLOW Pediatric Endocrinology The Department of Pediatrics, Medical College of Wisconsin, is recruiting a Postdoctoral Fellow to design and...
Hessner, Martin
GENE-ARRAYS@...
Feb 3, 2004 4:25 am
13047
You actually have too much RAM. It is a virtual memory problem on Windows computers with over 512 MB of RAM running Windows Me or 98. You must remove RAM or...
Hi, 15,000 genes is a bit large. You may want to make a subset of only the significant regulated , up or down , genes first and do the clustering on that...
Dear all, We have problems spotting from 1.5M betaine / 3xSSC with SMP3 pins onto Corning slide. We need to fit 20,000 spots onto a single slide and this means...
Hi Lev, the authors describe a buffer which results in very small spots so you can print up to 70000 spots per slide: Optimization of high-density...
JSobek
jens.sobek@...
Feb 3, 2004 11:59 am
13051
Dear Members I would like to introduce you to my website – www.medicalnewstoday.com It contains headlines of the latest medical news from around the world....
Dear Lev, As you know, numerous factors influence the morphology of microarray spots. These factors include the composition of the spotting solution, the...
Canel, Camilo
canelc@...
Feb 3, 2004 4:04 pm
13053
What type of pins are you using? We use 65 micron split tungsten pins and 25% DMSO spotting buffer and can spot ~50,000 spots on to UltraGAPs slides. SSC...