Hi Arraying Guy, Basically piezoelectric micro arrayers are quite well appropriated for protein arraying. They accept a bit higher viscosity and rinsing of the...
Dear Lev, We have occasionally seen spot-localized background on a variety of slide substrates. One solution we've used is a sodium borohydride pre-soak, as...
Successful printing on plain glass depends upon other factors, including which pins are used. My experience using a betaine+SSC buffer and Microspot 2500 pins...
Caprice Rosato
GENE-ARRAYS@...
Nov 2, 2004 7:28 am
16017
Hello Everybody, I am a research associate, working on protein truncation and solubility studies. So anyone who could give me some handy tips on generating...
Hi Teresa, The ANOVA approach makes no assumptions about whether there are a lot, or a few differentially expressed genes, since it tests each gene...
Tom Downey
tjd@...
Nov 2, 2004 7:28 am
16019
Not wanting to make this a manufacturers love-in, but the use of software assistance, such as SoftTouch technology on the MicroGrid products can also reduce...
Jeremy Clarke
jeremy.clarke@...
Nov 2, 2004 7:29 am
16020
Hi Xiuling, we routinely use a column-purification step to purify our PCR-products ( silica-gel based columns, for instance Qiaquick columns). That way you ...
Steinmetzer, Katrin
GENE-ARRAYS@...
Nov 2, 2004 7:29 am
16021
I'd like to know how do people approach the use of present calls when analyzing Affymetrix data. Is there an advantage of using only "Present" genes in the...
Monzon-Bordonaba, Fed...
GENE-ARRAYS@...
Nov 3, 2004 8:08 am
16022
Dear Monzon Calls is calculated based on the invividual probe intensity (for both perfect match and mismatch) using Wilcoxon signed Rank test. Absent calls...
Hello everyone, I am currently doing direct labelling of cDNA by synthesis from total RNA. I use SuperscriptIII from Invitrogen and Cy3/Cy5 dyes from Amersham....
In two color arrays, depending on your scanner and feature extraction software, there are additional flags for quality control besides only "A" and "P". Most...
The ESI built machines (which we have) have a separate platen for blot slides so there is no reduction in the number printed slides. These blot slides are...
mark.vanderhoek
mark.vanderhoek@...
Nov 4, 2004 8:13 am
16026
Hey guys, I am new to the group and just wanted to say hi! If you would like to check out a pic of me I took yesterday, or talk to me check my profile our over...
Bubiboo
alexander.fitch6985@...
Nov 4, 2004 8:13 am
16027
The Environmental Laboratory of the U.S. Army Engineer Research and Development Center is developing innovative technologies for detecting and assessing...
Perkins, Edward J ERD...
GENE-ARRAYS@...
Nov 4, 2004 8:13 am
16028
Successful printing on plain glass. My company specializes in producing surface tension modification equipment. We have faced this problem for the last 25...
Successful printing on plain glass depends upon other factors, including which pins are used. My experience using a betaine+SSC buffer and Microspot 2500 pins...
Caprice Rosato
GENE-ARRAYS@...
Nov 4, 2004 8:13 am
16030
Colleagues, A Computer/Bioinformatics Scientist position is currently available at DuPont (RES00205). If interested, please apply on line. Description ...
Jean-Francois Tomb
GENE-ARRAYS@...
Nov 5, 2004 8:15 am
16031
Matt, You may want to look at a pin technology that we sell that, among other advantages, eliminates pre-blotting all together. NanoPins are a capillary based...
bptexas2004
bptexas2004@...
Nov 5, 2004 8:15 am
16032
Matthew J Arno, Recognizing the risk of being identified as a microarray printing geek, I do proclaim that I have read all of the 'blotter slides' messages ...
AL BARI
GENE-ARRAYS@...
Nov 5, 2004 8:15 am
16033
Steve: I agree with you that without replication, present/absent calls have no much meaning (especially in the lower intensity range). However I think there...
Monzon-Bordonaba, Fed...
GENE-ARRAYS@...
Nov 5, 2004 8:15 am
16034
Thanks everyone for these helpful suggestions - it seems I need to find a blotter slide that will not allow blot/spot spreading too much. This is important as...
Hi there! I'm currently designing a custom array and have had previous problems with normalisation of my data, as most of the genes on my array are meant to be...
Dear all, I am looking for alternative suppliers of Cy3/Cy5 labelled UTP (not dUTP). I realise that many companies started to market fluorescently labelled...
Hello, You might find the following resource useful: http://www.changbioscience.com/faba/fabanoise.html It has lists of "stable" or "house keeping genes" as ...
I have replied to a number of emails in the last 2 weeks with no indication that the message has been posted. Am I doing something wrong. Bill Moffat ... Do...
Hi there, I am using an indirect labelling protocol. I make cDNA from total RNA and the couple the dye ester to a modified dUTP incorporated during cDNA...
Hello, Can you recommend any software for analysis of low-density microarrays of around 400 genes? The microarrays are nylon-membranes and the detection system...
We have had a recent problem with high background in the Cy5 channel. We see streaks and spots of intense red color. We use Invitrogen's labeling system and ...
Steve: I agree with you that without replication, present/absent calls have no much meaning (especially in the lower intensity range). However I think there...
