Sergios Artus claim that you can amplify RNA from 0.1 ng RNA starting material. see the following links. rather cofusingly there is also an arcturus also in...
Nicholas Lea
nicholas.lea@...
Feb 1, 2005 9:31 am
16429
Please delete the message "new 10 K user" ... From: B. Knebel To: GENE-ARRAYS@... Sent: Friday, January 28, 2005 2:56 PM Subject: new 10K user We...
B. Knebel
GENE-ARRAYS@...
Feb 1, 2005 9:31 am
16430
John, We have run QPCR assays on LCM. Most of our assays go down to the 10's of copies and all go at least to 100's of copies. The number of cells needed ...
Nancy S Shipley
Nancy.S.Shipley@...
Feb 1, 2005 9:32 am
16431
Dear Elizabeth, The Corning UltraGAPS slides work very nicely in combination with Operon's 70-mer oligonucleotides. Our customers prepare their oligo samples...
Canel, Camilo
canelc@...
Feb 1, 2005 9:32 am
16432
We generally capture about 100 cells using a Palm/Zeiss system. We've had nice results with as few as 30 cells. It's important to compare amplified samples...
Ana, You might check out the ABRF web site. They recently published a paper in Biotechniques on a study between oligo and cDNA arrays. Nancy Shipley, Manager ...
Nancy S Shipley
Nancy.S.Shipley@...
Feb 1, 2005 9:33 am
16434
Hi everybody, Can anyone tell me what's the most useful approach to evaluate label effect on gene expression arrays? I'm labeling a common reference sample...
Ana Arnizaut
arnizaut@...
Feb 1, 2005 9:33 am
16435
Hi Hanna, If you are looking to comparing RMA and MAS 5.0 expression intensities, it will not be similar as RMA normalization involves log transformation of...
Hi list, does somebody out here have already tested the incorporation level of Cys in the final cDNA before hybridization step ? I am trying to make this with...
Hi Olivier, I am not sure if this is a solution to your problem, but: With the Nanodrop, I never take the concentrations displayed on the screen, rather...
We have just published a LCM/QPCR study where we used 20 cells/rxn to assay for mRNAs in brain tissue. We also used immunocytochemical markers to choose the...
Michael_Poulter
GENE-ARRAYS@...
Feb 2, 2005 8:45 am
16439
Post this for a friend. Following equipment available at Northern California. 1. BioAnalyzer a. Bioanalyzer b. Vortexer c. Software d. Computer (optional) e....
Hi, Does anyone know an assay or kit for DNA isolation (and amplification) from a single cell?(to analyze DNA of a blastocyte cell with microarray)Thanks Duygu...
Hi, I hope you realise that the RT-enzyme does not incorporate the bulky Cy-dyes very eff.The solution is to add the much smaller amino-allyl modified nuc's in...
This is for those who are interested in plotting microbial genomes in circular plots. You can easily plot classification-type data or numerical data...
rohitghai_at
rohitghai_at@...
Feb 2, 2005 3:40 pm
16443
... This is possible by combining the test statistics for each gene. This is what we call meta-analysis and several papers have been published on this topic...
Adaikalavan Ramasamy
ramasamy@...
Feb 3, 2005 8:26 am
16444
Commercial posting OGT Services provide custom DNA microarrays. We currently have ongoing projects in the areas of CGH, Gene Expression, DNA-Protein binding,...
i'm new to this group.i'm doing my final year project in image processing of microarray images. can anyone explain me the cDNA probe preparation( PCR...
I work in a small core facility. We recently purchase a 5' biased 70mer oligo library to spot. We have had great luck with RNA samples, and are now working...
Kimberly Smith
kcpsmith@...
Feb 3, 2005 8:28 am
16447
Hi everyone, I am wondering if anybody here is using GeneTraffic DUO? I would like to know how to select the differentially expressed genes from the entire...
Hi Lev, Thanks a lot for you reply. I had just one more question about the way you quantify your Cys, which seems effectivly better than the (largely)...
Dear Saritha, http://www.bio.davidson.edu/courses/genomics/chip/chip.html Have a look at the above site Jai Prakash ... From: "saritha reddy"...
Jai Prakash Mehta
mehtaj2@...
Feb 3, 2005 12:15 pm
16450
Hi Kimberly: You need a kit that will allow you to add an amine modified UTP during transcription. The transcription product can then be easily dye coupled ...
You may want to look at this paper from Genome Biology 2005,6:R16 "Preferred analysis methods for affymetrix GeneChips revealed by a wholly defined control...
Megan-- How much sample you use for each sample in two color arrays is dependent on several factors, such as genome size and type of sample. When using total...
You can either use the Agilent labelling kit (direct labelling) or the AMBION amino Allyl MessageAmp Kit (indirect labelling). Both are working fine. Pascal ...
Pascal Barbry
GENE-ARRAYS@...
Feb 4, 2005 8:39 am
16454
Dear Kimberly, We also use the Arcturus RiboAmp Protocol, and from what I have read in the User guide it seems like you are actually producing anti-sense...
Calen Nichols
nichols@...
Feb 4, 2005 8:39 am
16455
RESEARCH TECHNICIAN Laboratory research technician positions are available in the Harvard Partners Genome Center in Cambridge, MA. These technician positions...
George Grills
GENE-ARRAYS@...
Feb 4, 2005 8:40 am
16456
Hi, We are thinking of buying a new (small) arrayer to print smaller dedicated arrays on the Nexterion MPX or Greiner HTA multi array slides. These slides ...
Paul Van Hummelen
GENE-ARRAYS@...
Feb 4, 2005 8:41 am
16457
Post Doctoral Scientist Department of Molecular Genetics Novo Nordisk A/S Denmark The department of Molecular Genetics now has a two year Post Doctoral ...
