Was this polypro or polystyrene plates? ... plates ... at ... were ... cycle ... were ... means ... the ... results....
t_martinsky
t_martinsky@...
Sep 1, 2005 7:08 am
17543
Hi everybody. I would like to establish a protocol for hybridization and washing of Agilent slides with the automatic hybridizer Lucidea ASP (Amersham). I have...
p66iacopet
paola.iacopetti@...
Sep 1, 2005 1:55 pm
17544
Dear Paola, You might want to try the AdvaWash Slide Washing Station to wash and dry your slides. The washing station has been tested for the Agilent slides,...
Hello guys, i have a question here. I have read a powerpoint file(Printing High Quality Microarrays) which wrote by Todd Martinsky(ArrayIt). In this file, he...
Dear Paola, for fully automated hybridization of Agilent arrays Tecan has designed the HS Pro series of hybridization stations. The systems automate the...
johannes_posch
johannes.posch@...
Sep 2, 2005 9:43 am
17547
Hello Everyone, Due to a number of IT upgrades currently underway, and the associated complications, the Gene-Arrays list will be down until Monday Sept. 12th....
Chandi Griffin
aniya@...
Sep 5, 2005 6:49 am
17548
DNAjobs.com Featured Jobs, September 2, can be viewed online at: http://www.genesciences.com/DNAjobsNews/2Sept05.htm...
hello,everybody! I'm a beginner of microarray.I am at a loss whether this instruments (VersArray ChipWriter CompactSystem) can be used to print my protein ...
Please feel free to comment OJB® Online Journal of Bioinformatics® Volume 6 (2) : 113-120, 2005 DNA microarray model for computation of absolute gene...
Has anyone else tried the Invitrogen Indirect Labelling kit with low elution spin cartridges? I'm looking for some feedback on yields, etc. Using 15ug of high...
Hi Kevin, my experience with the Invitrogen Indirekt Labelling Kit is the same (including the tech. staff). I´m using 20 µg total RNA, getting a yield of...
Hello Dr Crosby, I think that sending a description of your arrayer to this list will probably generate a few hits. If not here, then LabX.com is essentially...
Kevin, I'd start from increasing the recovery rate post coupling. 40% back from purification is not good, it should be somewhere over 70% at least. Also work...
Kevin, we have tested the Invitrogen indirect labelling kit in our lab, and we have obtained essentially the same results as yours, even from 20 mg of total...
Hi Kevin, Have you tried the new Pronto! Plus Indirect Labeling System from Corning/Promega? Typical yields from 5 ug of total RNA template primed with...
Canel, Camilo
canelc@...
Sep 8, 2005 1:14 pm
17558
Dear all, I am new in the field of genomics and microarray. So I am looking for general courses on this subject. Could anyone help me? Thank you for your...
Hi, Does anyone remember, about 2 years ago, a paper describing how a few hundred primers could be designed to reverse transcribe cDNA from all ORFs in one...
Hi Wang Yu We have been using Virtek Chip Writer Pro, marketed by Biorad Labs for couple of years. It is able to spot DNA/Protein and sugars or Carbohydrates....
Hi all, Has anyone been using VersArray Hybridization Chamber(BioRad)? I plan to buy some hyb chamber. My current experimental plan is to handle 8-16 slides...
Hi Anita We are using polypropylene plates from Genetix (x7022)and Corning's low profile high volume recovery(3672) for spotting our proteins/DNA and ...
I don't remember a B. subtilis paper but Talaat, Hunter et al had a paper in 2000 (not sure what journal) that described the labelling of M. tuberculosis RNA...
At my new core facility, we'll rely more on the investigator to produce spotting reagents and substrates. My first project will be printing short oligos...
Dear Masanao: I have no experience using the VersArray Hybridization Chambers and instead, have been using Corning Hybridization Chambers for years. My lab is...
hi, i would like to know if people use pronto plus system (promega) for total RNA isolation from soil samples. What do you think about the RNA quality for...
Dear Researcher, We are proud to announce our: 5th VIB MicroArray Users Group Meeting on November 16-18, 2005 in Gent (Belgium) It is a three-day meeting with...
Paul Van Hummelen
GENE-ARRAYS@...
Sep 13, 2005 6:38 am
17568
Deadline approaching: UNDERSTANDING THE GENOME microarray applications from the biology to the clinic II edition Sheraton Hotel Conference Center - Genoa,...
Ulrich Pfeffer
ulrich.pfeffer@...
Sep 13, 2005 6:39 am
17569
Hello all, I am looking for the best method for normalizing single-dye cDNA chips (Cy3). The arrays contain spiked controls at 5 orders of magnitude (although...
Paul Lang
paul.lang@...
Sep 13, 2005 6:40 am
17570
Hello, This is a test in the ongoing efforts sort out the problems with the new computer configurations and transfers being performed by the IT group. Thank...
Chandi Griffin
aniya@...
Sep 13, 2005 6:56 am
17571
Dear Masanao, we use the hybridization chamber HC4 from Quantifoil Instruments and we are very satisfied with it. We received very good results and the ...
