Hi I am doing DNA – DNA hybridization experiments using oligo arrays. I hybridization 2 test strains on one array and want to take the ratio of intensities...
Sabari Thirupathy
GENE-ARRAYS@...
Nov 2, 2005 8:59 am
17718
Hello All, Recently I hybridized few affymetrix arrays and I am struggling with its statistical analysis part. My experiment set up is very routine, like...
Jiang Miao
miaoj5@...
Nov 2, 2005 8:59 am
17719
Dear list, We routinely check the results of labeling reactions for Affymetrix arrays on a Agilent 2100 Bioanalyser. Normally we get a nice distribution of...
Hooiveld, Guido
GENE-ARRAYS@...
Nov 2, 2005 8:59 am
17720
Jim, don't know exactly how long they keep for, but when in doubt (say, if you haven't used them in a while), you can check if they still have good coupling...
Taboada, Eduardo
GENE-ARRAYS@...
Nov 2, 2005 9:00 am
17721
Hi Mark, We used the affy zebrafish oligo array to hybridize tilapia and got less than 5% present call. I am interested in the cDNA zebrafish arrays too, or a...
Johanna Craig
hannacraig@...
Nov 2, 2005 9:00 am
17722
Forgive me if this is a stupid question, but I am new to microarrays and new to this forum. I've been locked out of the room with the incubator, and in it, my ...
tomm_dogg
tomm_dogg@...
Nov 2, 2005 9:01 am
17723
Hello group I would like to produce a microarray from a cDNA library (>10000 clones) and have some questions that boil down to price and quality: How to decide...
Hi Thank you all for yuor sugestions regarding functional classification of genes. I'm looking for focussed cell cycle microarrays. I have checked up superaray...
Sheetal Bhan
GENE-ARRAYS@...
Nov 2, 2005 9:07 am
17725
Hi Guys, We use a universal reference standard with 2 colour microarrays and diseased versus healthy cells. Do you normalize such data by subtracting out the...
John McGregor
mcgregor@...
Nov 2, 2005 9:07 am
17726
Dear friends, I have a problem, and I'd want to ask you for some basic information about microarray. I will have a presentation on novel tissue and cell...
See comments inline below. ... If it is unpaired then use unpaired t-test. Furthermore you need to ensure that the assumptions of t-test (Gaussian distributed...
Adaikalavan Ramasamy
GENE-ARRAYS@...
Nov 2, 2005 9:08 am
17728
Hi Sankar, You can try using GACK described in the publication below: Kim CC, Joyce EA, Chan K, Falkow S. Improved analytical methods for microarray-based...
Thibaud-Nissen, Franc...
GENE-ARRAYS@...
Nov 2, 2005 9:08 am
17729
Free GACK installation is available from this site: http://falkow.stanford.edu/whatwedo/software/software.html . Darya. ... __________________________________ ...
Hi, I have 70-mer oligo arrays printed onto home-made polylysine slides. Just recently the chemistry has started to come off the slides after the hyb and...
Hi, I have been using Human cDNA array (19k and 8k)for microarray expts. I have scanned the slides using Genepix pro 6.0 and loaded the data in Gene traffic...
Hi Sankar, Francoise mentioned GACK by Kim et al (Genome Biology 2002) and I know many people who use it. Having said that, we have done lots of empirical...
Taboada, Eduardo
GENE-ARRAYS@...
Nov 3, 2005 8:56 am
17734
Personalized Medicine Program at Mount Sinai Medical Center is looking for a reliable technician to join our team, preferable experience yet not necessary are...
Greg Khitrov
greg.khitrov@...
Nov 4, 2005 7:43 am
17735
Dear colleagues Can anyone explain the following odd phenomenon. I have measured repeatedly measured the same sample of biotin labeled aRNA using the Nanodrop...
Moon, Lawrence
l.moon@...
Nov 4, 2005 7:44 am
17736
Dear All I found this inteesting microarray article on the web.. ...
Dear all, we are about to buy a new arrayer in our lab, and we hesitate between the Qarray2 from genetix delivered with a telechem head and SMP3 pins and the...
Dear all, I my looking for all sources of *Pseudomonas aerugionosa strain PA01* arrays (PCR or oligo, on Nylon or slide). Thanks for your help veronique ... ...
Hello erwan, In our lab we have a Qarray mini from genetix, and we are very satisfied by this machin and the aftersales service with proteigen is perfect. If...
Dear Lawrence, One explanation might be that the pH of your solution is changing in time. It is known that the absorption measurement is pH-dependent. Even if...
Ayoubi Torik (GEN)
Torik.Ayoubi@...
Nov 7, 2005 9:43 am
17741
Dear Lawrence, If you would like to share the data with us you are welcome to do so. It may help to resolve the issue. I would imagine that a 10ul solution...
David Lach
GENE-ARRAYS@...
Nov 7, 2005 9:43 am
17742
Dear all, I want to determine the degree of coexpression of the identified homolog pairs during seed germination (I only adopted three time points) of the two...
LI LIN
GENE-ARRAYS@...
Nov 7, 2005 9:44 am
17743
Dear all, I want to determine the degree of coexpression of the identified homolog pairs during seed germination (I only adopted three time points) of the two...
LI LIN
GENE-ARRAYS@...
Nov 7, 2005 9:44 am
17744
Hi Lawrence, One possibility is that your RNA is degrading into free nucleotides over time. Free nucleotides absorb higher at 260 nm than intact RNA and that ...
Hendrickson, Jill
GENE-ARRAYS@...
Nov 7, 2005 9:44 am
17745
Was the same actual drop? Or was it separate aliquots? If it was the same drop maybe it could be evaporating on the machine... Not sure why plasmid DNA...
Lawrence, I've had somewhat similar experiences but found that if I heated the RNA at 55 C for a few minutes that it stabilized. I'm guessing there's just some...
