Post-doc Position in Data Mining/Bioinformatics The University of Texas at Dallas Richardson, Texas http://www.utdallas.edu/~ying.liu The Laboratory for...
yingliu_utd
yingliu_utd@...
Dec 1, 2005 8:44 am
17839
Dear Boris, You should have a look at Tecan's HS Pro series of hybridization stations. There are 2 systems offering throughput of 1 - 4 slides (HS 400 Pro), 1...
We are labelling amino-allyl labelled cDNA with Cy3 & Cy5 NHS esters using a standard indirect labelling protocol. We have tried a combination of purification...
Looking for sharp individuals with proven experience in microarrays and microarrays scanners. Suitable candidates living in (or willing to move to) Southern...
If you are a good bio-informatician and you want to work in the Netherlands, there is a 2 year position open in Amsterdam at the Netherlands Cancer Institute. ...
Hey Kelly, In the past when I did labelling experiments, I used microcon filters to clean up my sample. They're pretty easy to use and I never had any problem...
Kelly, You can try just use QiaQuick column but wash two times at extra wash buffer. The purpose of the extra washing is to wash away free dyes remain on the ...
Dear Sir, I have experience of design microarray sanner and manufacture the microarray products.Please feel free to contact me. juntingl@... Thanks CP...
Hi Kelly We use Qiagen RNeasy mini columns for cleaning up our labelled cDNA (and also for cleaning up the unlabelled cDNA after cDNA synthesis). Hope this...
Boris, I just started using Series M Lifterslips from Erie Scientific with good results for a 30K+ oligo array on ultragaps slides. I find the thicker Series M...
Hi Melissa, Did you use Microcon filters for dye purification? Which size filters did you use? I've used the YM-30 filters for cDNA purification (great...
For the past two years we are doing hundredrs of microarray and using Roche microarray purification kit, it has two components 1. cDNA purification kit 2....
Dear Madam/Sir, ExactAntigen is organizing the tissue arrays available from both academic and commercial resources (http://tissue-array.exactantigen.com/)....
hanqingx
hxie@...
Dec 5, 2005 12:18 pm
17851
Dear Boris and Jesse, we have hybbed succesfully on Corning slides and on Poly-L-lysine slides with Lifterslips for many years using Telechem cassettes. We...
Could someone please tell me what a genotype microarray is. ASAP! I have been able to find very little information on it and I am quite curious as to how it...
Hi All, I'm new to microarray analysis. Wondering whether you could advise me how to perform the analysis on some Affymetrix data. The data includes: 6...
Winston
GENE-ARRAYS@...
Dec 6, 2005 1:28 pm
17854
Hi, We are currently just about to print a custom array but we are not sure of what slides to use. We want to use unmodified 40mers and we are thinking about...
Good afternoon, I have slides that printed 3/4 of the samples fine but the last 1/4 certain spots are much smaller than normal. This can be observed on every...
Fiona McIntosh
GENE-ARRAYS@...
Dec 6, 2005 1:29 pm
17856
We are using a non sequenced plant species but have data from a superSAGE. This means we have 26 bases of the 5000 genes. If we synthesize the 26bases on a...
Nancy Podevin
GENE-ARRAYS@...
Dec 6, 2005 1:31 pm
17857
Dear Boris, QInstruments has developed a very easy to use cover slip technology called DiscoverSlip. The DiscoverSlip technology improves performance of...
You can try this link: http://search.atomz.com/search/?sp-q=genotype+&sp-a=sp1001209f&sp-f=iso-8859-1&scope=s&x=0&y=0 Martin ... -- Martin J Wang WIS Biomed ...
Oligo or cDNA array? ... From: Share info on GeneChips tech and Microarrays in general on behalf of Nancy Podevin Sent: Wed 11/30/2005 5:10 AM To:...
Paul Lang
paul.lang@...
Dec 7, 2005 9:47 am
17860
Hi, Fiona, Are the smaller spots the last spots to be spotted? If so, is there any humidity control for your microarrayer? From my experience, after 2 ~ 3...
Ye, Bin
GENE-ARRAYS@...
Dec 7, 2005 9:47 am
17861
As a place to start troubleshooting, I suggest mapping the small spots back to the sample locations in the source plates. Examine the wells of the poorly...
Todd Martinsky
todd@...
Dec 7, 2005 9:48 am
17862
Hi Fiona, I don't know which arraying system you are using, but assuming you are carrying out contact printing, my first suggestion would be that you are ...
Jeremy Clarke
jeremy.clarke@...
Dec 7, 2005 9:48 am
17863
Dear Winston, Take a look at www.bioconductor.org Search for affy package in their. If you will have a doubt, please, on bioconductor site there is a mailing...
Marcelo Luiz de Laia
mlaia@...
Dec 7, 2005 9:49 am
17864
Fiona, I see this in samples which contain only buffer (buffer blanks). Caprice . . . . . . . . . . . . . . . . S. Caprice Rosato...
Caprice Rosato
GENE-ARRAYS@...
Dec 7, 2005 9:49 am
17865
... Hi Sergio, epoxy slides are the first choice for printing oligos, because of higher binding capacities due to active linkage chemistry. The epoxy group...
I have cDNA microarray raw data in tab delimited text file. Basically I am comparing infected and uninfected tissue after pathogen infection in tomato. For...
hi, all. I have spotted my probes on microarray slide of Nuricell. I obtained a bad result that each different spot diameter I want to your comment. ... ...
