I have check some printing buffers for the epoxy slides. But the signal is not very good. Anybody took some better or efficient buffer for the epoxy slides?...
Hello, I like to download a relatively complete gene list of human transcription factors in HUGO symbol format like NFKBIA. Can anyone suggest one website for...
Hi All, I have done an microarray experiment on samples that were cleaned with Qiagen RNeasy kit without the optional DNase treatment. The optional treatment...
Dear all we are currently working with an inhouse microarraying machine using split-pins of different suppliers (Genetix, Telechem). The organic components to...
j_zerweck
j_zerweck@...
Aug 2, 2006 11:03 am
18586
Hi Krzysztof, I have not tried treating with DNase, and although doing the treatment on Rneasy columns should eliminate any potentially interfering salt ...
Dear JZ, Have you tried sonication? Genetix usually recommends sonication in a detergent solution (like their aQu Clean), followed by sonication in filtered...
If you do decide to use Poly-Lysine coated slide please be careful, the poly-lysine is only bound to the slides by an electrostatic charge, and can come off...
stevefhawkins
stevefhawkins@...
Aug 3, 2006 7:56 am
18590
Hi Krzysztof, I strongly recommend to treat your samples with DNase I. You may use Ambion DNase I. If a protein carry over is a concern use phenol/chloroform...
Hello, I routinely use the Qiagen columns, and always do the optional DNAse (it's something like US$ 0.25 per reaction, so it's worth it to me). In the past 4...
Hi Li, Epoxy slides are very hydrophobic, so that when you spot onto them with normal aqueous buffer there is not much transfer of solution to the slide...
stevefhawkins
stevefhawkins@...
Aug 3, 2006 7:57 am
18593
Krzysztof, you may want to consider joining another Yahoo group: qpcrlistserv...there are alot of qPCR experts there and loads of information and opinions in...
Do I have it right that you are printing compounds and not biologic material? I wish you very good luck in this endvour!! One thing you might consider is...
The only problems that I have heard of with using older chips have to do with loading the hybridization cocktail--the septa may become cracked or otherwise not...
... I tried to use epoxy slides with phosphate buffers and I also found the signal is not very good so I tried Pronto buffer from Corning and got excellent...
sergio_barberan_soler
sergio_barberan_soler...
Aug 7, 2006 11:24 am
18597
Dear all, What method(s) are used normally to discriminate the single-base mismatch and other nonspecific binding in oligonucleotide microarrays? Washing them...
Dear Sir, I am working in non-affy whole genome spotted oligo arrays with one color channel. I wonder whether you could provide suggestion and/or refer related...
orchid3886
orchid3886@...
Aug 7, 2006 12:15 pm
18599
Dear members, I have a problem with high red background. I am using spotted arrays. I think I am talking all possible care, some of them are as follows 1)...
... If you're coating your own and you want high quality substrates you'd then better start with very very clean glass. We didn't want to use pirannha...
... signal ... the ... We use a sodium-phosphate & sodium-sulfate buffer (SPSS) with corning epoxy-silane slides and are satisfied with its performance. How...
... arrays. I think I am talking all possible care, some of them are as follows ... centrifuging the samples for 30 minutes. ... bubbles or gaps after 18 hrs...
Hi Jenny, Trisha, Group, We recently ran a project where the customer had about 10 MOE430A GeneChips that were 12-18mo past expiring and insisted on using...
Johns, Christopher
johns@...
Aug 8, 2006 10:52 am
18604
... The food coloring won't be necessary if your printing buffer contains a salt; in that case you could see the spots under the microscope. If you're not...
We have used 3X SSC/0.75 M Betaine with much success. If you are doing a long run the evaporation from the plates seems to be mitigated with the Betaine. ja...
... We use indirect labeling of cDNA and we use the Invitrogen SuperScript microarray labeling kits. I highly recommend them, however I am personally...
I once had this problem of high red background and traced it back to the use of an old 10% SDS solution. I now make up 10% SDS more regularily and keep it out...
Davies, Dr R.
rd9@...
Aug 8, 2006 8:47 pm
18608
... By the way, there are published protocols for coating with silane or epoxy-silane. I Googled them once but we decided to just buy commercial microarray...
BIOTAQ(TM) Newswire August 8 2006 To learn more about BIOTAQ's List Service please visit: <http://www.biotaq.net> http://www.biotaq.net For further...
BIOTAQ Newswire
services@...
Aug 8, 2006 8:51 pm
18611
Hi, Can anyone recommend a good source of deionized formamide for hybridization? Also, my hybridizations were working but have recently stopped looking so...
