hello i am pavan, presently we are not using any of agilent products, we are based on genomic solutions platforms, and we are using glass arrays from ocimum,...
hello everybody i am working on gene expression profiling on lung cancer. my problem is the sample, I am working on Fine Needle Aspirate (FNA) Samples from the...
Thankyou, That was helpful Babru Samal <babru02@...> wrote: Look here for some info http://www.genetools.us/genomics/Microarray%20software%20catalog.htm ...
what all techniques do you use, do you want any improvement in present clustering techniques manulemalin13000 <no_reply@yahoogroups.com> wrote: Hello...
Dear Pavan, Have you looked at the Illumina whole Genome arrays.The pricing is really amazing and the labeling cost is a fraction of any other method. Cheers ...
Summer Training with Project - Bioinformatics & Cheminformatics. (Molecular Modeling & Computer Aided Drug Design) Medwin Hospitals, a Multi Speciality...
hello groupmates, have any one of you isolated rna from liver tissue. if yes, did you face uncommon resolution of extracted total rna bands on rna gels, i mean...
Hi I don't know if this will help but you may want to try it. Lucille Personal communication from QIAGEN I just wanted to bring one important note to your...
Rainbow, Lucille
lrainbow@...
May 9, 2007 11:20 am
18971
Hi, I am not sure about "uncommon resolution" that you mentioned. I used Agilent Bioanalyzer to check RNA quality. My experience is that sometimes it seemed no...
Hi, I am not sure about "uncommon resolution" that you mentioned. I used Agilent Bioanalyzer to check RNA quality. My experience is that sometimes it seemed no...
Hello Dr Lucille, Thank you for the suggestions, i was aware of the high glycogen content of liver tissue, and i was thinking to use lithium chloride 4molar to...
Hi, thank you for the reply, and what i mean by uncommon resolution of liver rna bands, is that tthey run very fast, equivalent to 100 to 200 base pair dye...
Hello, Pavan, I use Trizol to extract RNA, and homogenize tissue by a homogenizor. During handling tissue or homogenization, keeping low temparature is very...
I would suggest when using trizol to extract be very careful to leave enough overlay on the interfacial layer. That should help prevent protien contamination....
hai, thank you for the reply, even i use trizol, homogenize my tissue using mortar pestle, in liquid nitrogen, and yes i try not to thaw the tissue. and i...
Hello group, Until now we are using cDNA arrays from Stanford. Now we have to switch over to Oligo arrays since they stopped the production of cDNA arrays....
I am trying to find a company that can OEM a massively dense microarray described as follows: Custom make a massively dense microarray for sequencing the human...
wink823@...
May 16, 2007 7:32 am
18981
hello mahesh, oligo arrays work well go for 50 to 60 mer oligos, protocol remains the same, isolation - amplification - labelling - fragmentation -...
... How are you going to sequence a vertebrate genome with 16x10^6 features on an array? I'm just curious... Maybe you don't mean sequencing in the same way I...
Hello, I'm using a multi-species microarray generated using at least three tissues, and so hybridization only occurs on roughly 1/3 of the features at best (at...
Dear group participants. I'd be very gratefull if you could share your impressions and comments about Chip-to-Hits events. Do you find them helpful for...
Dear, I am new in microarray. I want to know about any web site which provide free basic information about microarray or free books about microarray. Secondly,...
Anyone notice any problems with the autoloader of this machine? Turns out that the vibrations during scanning eventually knock off the connectors to the...
Dear Nauman: We have a free electronic library of microarray publications as well as many protocols on our website. Please see www.arrayit.com Kind regards, ...
... http://bsu.ncl.ac.uk/downloads.php?cat_id=1 One of the links on there is a full free book for microarray data analysis from CSC Biobox. A quick Google...
There is lots of free information on www.Lab-on-a-Chip.com . They also have a forum where you can ask questions too. You just missed an introductory course : ...
selectsystems2000
selectsystems2000@...
May 26, 2007 5:16 am
18992
This is much better meeting (just ended). It will be in Barcelona next year. www.microarraytechnology.biz Regards, Ken...