Hello all, I am having problems printing with our ArrayIt SpotBot printer and I'm hoping someone out there with the same system can provide some help. The...
The answer lies in the surface preperation of the array. With old fashioned chemical treatment the technique was to heat the array to drive out all moisture...
Dear Ann Marie: Spotting can be more erratic if deionized water is being used in the pin washing system of any microarrayer, including the SpotBot. If you're...
Todd - What is the logic behind / basis of this water effect? I've never had this problem with my aQu pins (using milliQ for all system and wash solution) -...
Nigel Saunders
nigel.saunders@...
Jul 9, 2007 6:53 am
19036
Dear Nigel: The basis is oxidation, or the loss of electrons from the metal in the pins. Since 18 mega Ohm water has no electrons, it will want to pull them...
Dear Colleagues, We bought a used Genomic Solutions MicroArray Scanner (GeneTac 1000 S/n 100018) but without cord and computer. If you could help me to make...
Gabriella Fabian
fabian.gabriella@...
Jul 10, 2007 8:34 am
19038
... You might also want to try posting this on http://www.labx.com/ which deals with used lab equipment and software. It might also help to know what the...
Dear Todd, Does this water effect apply to the sillicon pins (Parallel Synthesis)? Cheers, Sastra Chaotheing BIOTEC Protein-Ligand engineering and molecular...
Hi, I'm new to the microarray listserv. I'm having a problem with uneven hybridization signal on our yeast DNA microarray slides. I've posted some pictures on...
Hello Everyone, Does anyone have suggestions of a person they may know who has extensive microarray and PCR experience with creating tests (prenatal genetics,...
I've been asked by my University to try and estimate usage of agriculturally-related microarrays. It is part of justifying the existence of our Core Facility,...
if the coverslip falls off easily in wash, then it has not dried out. how are you applying the hybridization cocktail to the slide...that could lead to a...
It looks like the distribution of the hybridization solution is not even. If you have access to any other hybridization chambers or automated systems you...
I have a suggestion. After you set up the chamber, before you put it in water bath for overnight or 16h , heat a block at 65, place the chamber on block and...
For even hybridizations try the SlideBooster: http://www.implen.de/s_booster01.htm Due to agitation during the hyb and the even setup the system is best suited...
Hi Dr. Dahlquist, We are the Microarray Core Facility of University of South Carolina. We have three different Hyb systems at our facility (GeneTac, Agilent...
Kam, In regards to the uneven signal across your slides, it could be in your post-hyb washing steps. I used to have a similar problem with my wheat long oligo...
Dear Peter I am responsible for microarray applications at the Centre for Proteomic and Genomic Research (CPGR), a core facility based in Cape Town, South...
HelloDan, Thanks for the idea of labx! Gabi ... after it? ... Mon/Wed) ... Thu/Fri)...
gabriellafbn
gabriellafbn@...
Jul 20, 2007 8:19 am
19052
Dear all, We have a Genepix 4000A scanner and would like to have it checked by a qualified technician. The scanner is working fine, but it had no maintenance...
Hello all. I am new to the microarray world and have some basic questions. I have a huge data set of microarray results from GE Codelink arrays. Each treatment...
Hello everyone I am new to microarray. I have recently printed oligoes of 30-70mer on Poly l lysine coated slides. I will use Genomic solution Hybstation for...
Hello every one I have recently printed 30-70 mer oligoes on Poly L lysine coated slides. Now can any one help me about the hybridization protocol (manual)....
Dear Bushra Ijaz, Assalamoalikum, I am from Pakistan and doing Ph.D. from Chinese Academy of Sciences, China. From which country do u belong and where r u...
Dear all, I would like to follow up on my email to the list about two weeks ago, regarding maintenance of our GenePix 4000A scanner. Since that I received some...
Dear all, I would like to follow up on my email to the list about two weeks ago, regarding maintenance of our GenePix 4000A scanner. Since that I received some...
Research Analyst - Microarray core leader, Johns Hopkins University School of Medicine Note - this is not an official job posting. It is a request for...
Dear Colleague, This letter is to make you aware of the upcoming 2008 Conference of the very successful 'Genomes to Systems' series. It will be held at the...
