Many thanks for all the comments and advice. They are very useful. Regards, Sastra ... problems with dots falling of. My company manufactures surface tension...
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Sep 6, 2007 11:32 am
19071
Dear all, has somebody already used the Geniom platform ?, on paper it seems to be the answer of ALL yours problems in your lab. I would like to discuss with...
Respected All, Yesterday, we were isolating Genomic DNA from Chicken's blood and I came across a strange step in the protocol which told me to add Proteinase K...
Dear Tarun, In our lab we work routinely with DNA extracted from FFPE material for subsequent labeling and hybridization on BAC arrays. We also use a...
qPCR NEWSLETTER - September 2007 – qPCR 2007 talks online If this newsletter is not displayed correctly by your email client, please use following LINK: ...
Hi all We are about to buy a microarray scanner and a real-time PCR thermocycler. Does anybody have any experience in using the Chromo 4 system of detection of...
About qPCR: qPCR also known as Real-time quantitative PCR has rapidly developed as the prime technology to detect and quantify nucleic acids in complex...
ejaladi
ejaladi@...
Sep 26, 2007 5:29 am
19077
Hello all, I am a post-doc at Washington University in St. Louis. I am familiar with the nanodrop spectrophotometer for dye incorporation measurements. Is...
Hello to every one. I want to know do any one use codlink human whole genome chip. I want to ask can we hybridize single chip with cy3 and cy5 labeled cDNA...
Dear yahoo groups readers, I would like to make an open call to prospective applicants for two open job positions, one in bioinformatics and one as a wet lab ...
I am using Affymetrix GeneChip system to detect mouse gene expression profiles in developing embryo. As you all know, Affymetrix software MAS 5.0 provides ...
try dchip. it also gives calls www.dchip.org well i don't rely on absent or present calls as a good estimate. microarrays are normally not that sensitive....
Hi everyone, We are hoping to trial one of the new Maxwell 16 automated DNA/RNA purification systems for DNA/RNA purification (http://www.maxwell16.com). From...
We've been trying to print a MoAb to a peptide hormone on slides with a variety of surface chemistries (epoxy, amino, polystyrene, etc). Generally, we get...
To the best of my knowledge the SDS denatures the proteins so that they can be attacked by Proteinase K. Obviously Protienase K is not denatured. sgar...
technologene
sgar@...
Oct 18, 2007 7:07 am
19086
Hi, For my microarrays I used oligodT priming of RNA for cDNA synthesis. Now I want to follow up with qRT-PCR, and I notice that in the kit I am using...
Hi, I'm just starting a new experiment. I plan on isolating 5 different types of cells from one tissue and then treating each with a compound. For each...
Hi Sheryl, I am assuming that you going to use two color labeling. You need to consider how you are going to compare the arrays between themselves. This can be...
Hi Sheryl: I would suggest that you optimize the oligo arrays with some high quality RNA from your tissue before you begin your experiment. Then, start...
Hi Folks -- We have a very lightly used VersArray ChipReader (5um, confocol laser scanner) and NEVER-used VersArray ChipWriter Compact microarrayer that we...
Hi, I am looking for available fungal microarrays (filamentous fungi only). Does anyone know where to find them? Thanks Francois Francois Piumi Laboratoire de...
Hi Francois, If you have the time and the skills for an oligo design on your favorite organisms, Agilent provides custom arrays for a very reasonable price. ...
devaux@...
Oct 31, 2007 8:04 am
19094
dear all, I am looking for available Corynebacterium diphtheria microarrays. Does anyone know where to find them? Thanks veronique -- Veronique Le Berre ...
Hello Dr. Piumi, We have microarrays of Aspergillus Fumigatus available for the very reasonable price of ABSOLUTELY FREE. The National Institute of Allergy...
Hello microarray colleagues: I'm promoting my microarray blog. I'm optimistic you'll find some of the content interesting. http://arrayit.blogspot.com/ Feel...
Dear Listserver Subscriber, The ABRF Microarray Research Group (MARG) invites you to participate in the 2007 ABRF Survey - "2007 MARG Survey". This survey...