Dear Agnes: I wrote a blog entry on this topic. You may find it useful. http://arrayit.blogspot.com/2007/10/microarray-hybridization-manual-versus.h tml Kind...
Hi Brian, Many thanks for your help, and yes, it makes perfect sense. At least I believe I got it, which is quite something - the last time I felt I understood...
Hi Lev, I have not been able to locate any hard numbers for this specifically but, the 5' amine terminus is an aliphatic amine while the exocyclic amines on ...
Brian Ward
Brian.Ward@...
Feb 1, 2008 8:13 am
19151
Hi, My lab has recently been given an old Molecular dynamics Array Scanner Generation iii microarray scanner. Unfortunately it did not include a computer to...
Hi friends, Could you please tell me how to estimate the missing values in microarray data? I came to know about "impute" package which requires "R"...
Hi Everyone, I know that Gene Traffic by Iobion is no longer in production, or being supported, however, we still like using it for its project management,...
Generator Microsoft Word 11 (filtered medium) Dear Colleagues, Scientific Services at The Jackson Laboratory (http://www.jax.org) is conducting benchmarking...
Sonya Kamdar
sonya.kamdar@...
Feb 6, 2008 4:09 pm
19155
Agnes, At Signature Genomic Laboratories we have automated the washing procedure of our arrays by utilizing the Little Dipper and NoZone workstation. Our...
kylersundin
kylersundin@...
Feb 7, 2008 9:17 am
19156
We use HEEBO micro arrays to examine gene expression in human cells. We use the indirectly method of labeling the mRNA, i.e. aminoallyl dUTP incorporation...
Davies, Dr R.
rd9@...
Feb 8, 2008 12:14 pm
19157
Dear Dr. Davies: Do you Cot-1 in your hybridization buffer? If yes, I would suggest removing it and trying again...it's possible it's blocking the...
Hi Reg, From our experience, the most common cause for array and RT-PCR data disagreement is due to the probes for each platform actually measuring different...
Christopher Barker
GENE-ARRAYS@...
Feb 13, 2008 5:44 pm
19160
Hello Reg, Iım not sure about your specific gene of interest, but is it possible that your two assays are measuring different parts of the gene? Alternative ...
Hi, Check out the all new microarray data analysis webservice http://geneincense.com Looking forward for your precious review and referrals. Kindly do login...
Check out the all new microarray data analysis webservice http://geneincense.com Looking forward for your precious review and referrals. Kindly do login and...
Hi Reg, I totally agree with Chris. To our experience, qRT-PCR is always more sensitive than microarrays. Realtime PCR is normally designed with short ...
Ying, Jun
jun.ying@...
Feb 14, 2008 7:58 am
19165
Hi, I am using Genespring 9 but I have a whole Agilent platform. If I can help... I am looking for help about statistics tests integrated with this new ...
hi, Dont get tooo frustrated . Contact this technical support team for genespring 9.0 through this email informatics_support@... they will help u... ...
The migration to a new software version usually entails some preparation indeed. With respect to the GeneSpringGX application, there are two scenarios ...
Duval, Manuel
GENE-ARRAYS@...
Feb 15, 2008 9:07 am
19169
Hi Jun, I am interested to learn more about your experience with degraded RNA in real-time PCR. How did you measure RNA degradation (using 28S:18S ratio, or...
Agnes Azimzadeh
GENE-ARRAYS@...
Feb 15, 2008 9:07 am
19170
Hi everyone, I have the same problem. Apparently this new version has been developed to easely deal with Agilent FE software, but it is quite complex to work...
Javier Margareto
j_margareto@...
Feb 15, 2008 9:08 am
19171
It's always possible the RT-PCR and the oligo on the chip are detecting alternative splicing, but before getting too much into the complexity of the geneset,...
Agnes and Jun, Degraded samples such as formalin fixed, paraffin embedded (FFPE), or very degraded samples for whatever reason have been found to be very ...
Eric T Blair
GENE-ARRAYS@...
Feb 16, 2008 8:17 am
19173
Hi Reg, I have never worked with HEBBO arrays but I have worked with a number of different platforms and have seen quite often that real time and arrays do not...
Agnes, The experiment was performed by another person in our lab several years ago. RNA was incubated with RNase for various length of time. RNA quality was...
Ying, Jun
jun.ying@...
Feb 16, 2008 8:17 am
19175
Peter, Can you suggest alternative Hyb conditions/buffers then? What's your favourite? Do you think that all oligo arrays behave this way, as opposed to cDNA...
Hi, I am interested to learn about available databases for Copy Number Variations in cancer. I am not looking into a particular cancer (or a particular...
Noushin Farnoud
GENE-ARRAYS@...
Feb 21, 2008 8:14 am
19177
http://www.google.co.uk/search?hl=en&q=%22copy+number+variation%22+%2Bdatabase&btnG=Google+Search&meta=&aq=f ... -- Bioinformatics Support Unit ||...
