Hi, Illumina has some arrays like these Regards Philippe Guardiola, MD ... De : Paola Iacopetti <paola.iacopetti@...> A :...
phguardiol@...
Mar 3, 2008 9:55 am
19212
Is the housekeeping gene, here cyclophillin, a real housekeeping gene? If your treatment for sure not affects its expression, my answer is yes. Otherwise, use...
Jingping Yang
GENE-ARRAYS@...
Mar 3, 2008 9:55 am
19213
Hi all, I am having problems with data generated from Affymetrix Gene Array. Most targets identified as up- or down-regulated for more than 2 folds are postive...
Tao Liu
GENE-ARRAYS@...
Mar 3, 2008 9:55 am
19214
Hi Reg, There are a few ways, and the good news is that you can if you want even do some of them after the hybridisation so that you do not have to sacrifice a...
Hi Reg, The facility that printed the array may have done a QC hybridization to check the lot of arrays printed. They may have an image of a slide hybridized...
markband@...
Mar 5, 2008 5:46 pm
19216
Hello, You may use a SYBR gold staining to check whether the oligo is printed or not. I hope this may help you Regards Guillermo _____ De: Forum: microarray...
Dear colleagues, We are interested in a complete oligo set for mouse for in-house spotting. Which company can you recommend? An alternative solution could be...
Ludwig Eichinger
GENE-ARRAYS@...
Mar 6, 2008 5:47 am
19218
Hi Tao, The first thing I would do was to check all of QC indicators to see how your RNA has performed overall. I would suspect the source sample to be a...
Hi All, I am trying to pool Affymetrix experiments(U133a and u133plus) from GEO and trying to put up as 1 group. Reason being that in any one experiment , my...
Jai Prakash Mehta
mehtaj2@...
Mar 6, 2008 5:48 am
19220
You can't pool U133A and Plus 2 data and don't try. We published that the U133A and the U133 plus 2.0 arrays produce different measurements for the same...
Enkemann, Steven
GENE-ARRAYS@...
Mar 7, 2008 5:48 am
19221
Mark, Many thanks for your comments. We do need to have a look at QC. Reg ... From: microarray@yahoogroups.com [mailto:microarray@yahoogroups.com] On Behalf Of...
Reg Davies
rd9@...
Mar 7, 2008 5:48 am
19222
HI Ludwig, We ordered our oligos from Qiagen for our "Toxarray" chip here at Health Canada. That was several years ago so I'm not sure if they are still in...
Lynn Berndt-Weis
GENE-ARRAYS@...
Mar 7, 2008 5:48 am
19223
HI Steve, That is interesting information i didn't know of! When quickly going through your paper (pubmed id 16776839) i noticed you analyzed the 2 plus array...
Hooiveld, Guido
GENE-ARRAYS@...
Mar 8, 2008 8:28 pm
19224
Hi Jai, I agree with Steven, keep the experiments separate for all the normalization and initial analysis steps. Once you get the gene lists from the two sets...
To Whom It May Concern: My name is Thomas Ahn, and I work for an independent research firm, Guidepoint Global, which is based in New York. Our company has a...
We have a client who need some U133A arrays. I am wondering if anyone out there might have some on there hands or anyone need to use the same arrays that can...
Haiping Hao
GENE-ARRAYS@...
Mar 12, 2008 8:21 am
19227
Dear all, we are planning a test experiment of hybridization on an Agilent custom array. They offer different designs: e.g. 4 x 44K, 8 x 15K etc. If we...
Enrico Carlon
GENE-ARRAYS@...
Mar 12, 2008 8:21 am
19228
Hi there everyone, Our lab recently upgraded to a piezoelectric printing system and we settled on the Scienion SciFlexarrayer S3. I am wondering if any of you...
Orfe, Lisa
lorfe@...
Mar 12, 2008 8:21 am
19229
Hello, Ocimum has good cancer chips, but on glass substrate. Paola Iacopetti <paola.iacopetti@...> wrote: Hi everybody, I am looking for...
Tuesday, March 11th, 2008 Biomarkers in Drug Development Last phase of registration 9th-10th April '08, London, UK Thanks to a fantastic lined-up of speakers...
ACI Europe
mmulazzi@...
Mar 12, 2008 8:21 am
19231
Hi, it will be eight times the identical microarray on the slide. But this microarray is made according to your individual selection of 15k spots (there will...
Neuschaefer-Rube, Olaf
oneusch@...
Mar 13, 2008 12:21 pm
19232
Hi Enrico, Since Agilent synthesizes each oligo in place based on the computer file you provide, they can make any combination of arrays on a single slide. So...
Christopher Barker
GENE-ARRAYS@...
Mar 13, 2008 12:21 pm
19233
Dear group members Please pass on this message to any individuals who you think might be interested. A CV and covering letter should be sent to ...
HI Olaf and Enrico, We routinely work with Agilent custom arrays. You do need to print 8 identical arrays on 1 8 x 15K slide (unfortunately , they won't let...
Andrea Barczak
GENE-ARRAYS@...
Mar 17, 2008 8:21 am
19235
Hi, Just got the information from Agilent that there is now no minimum order number for custom arrays any longer!!! Olaf Neuschaefer-Rube University of Essex,...
Neuschaefer-Rube, Olaf
oneusch@...
Mar 17, 2008 8:21 am
19236
Hi there, Do you have experience making cDNA from bacteria RNA? What kind of primer do you use? If random hexamer does not work well, how long the primer to...
Jing Zhang
jzhang02@...
Mar 17, 2008 8:21 am
19237
Hi Jing, If you're not too bad in french you can use "FindExpress", a software created to design a set of specific primers for your bacterial RNA. You can...
BOSC 2008 Call for Abstracts The 9th annual Bioinformatics Open Source Conference (BOSC 2008) will take place in Toronto, Ontario, Canada, as one of several...
darin.london@...
Mar 19, 2008 8:31 am
19239
Dear collegues, I try to follow several ways to get more (publishable) confidence in my microarray data. Among others, I used the rank product FDR approach ...
Neuschaefer-Rube, Olaf
oneusch@...
Mar 19, 2008 8:32 am
19240
Please post the following job announcement to the list-serve. Thanks, Bart Frank, PhD Functional Genomics/Microarray Specialist. A position is available at the...
