please delete me from the list ... From: <microarray@yahoogroups.com> To: <microarray@yahoogroups.com> Sent: Saturday, December 29, 2001 2:22 PM Subject:...
Dear friends. Did you have an experience to scan the same slide by different direction. We were analyzing the dyeswap data and I found out the trouble from cy3...
Henrik, thanks for sharing your insight on the subject. ... have the maximum order preserving set of oligo intensity ... This is ambiguous. For instance, the...
jacobsen
jacobsen@...
Jan 4, 2002 3:10 am
6325
Hi, All: Happy New Year to all! Does anyone know how to keep the short oligos from degradation? How long it may be in good situation? Any comments and...
Xiuling Wang
GENE-ARRAYS@...
Jan 4, 2002 3:19 am
6326
Hello, Can anyone who is using commercial hybridization chambers and/or automated washing stations for processing of slides please tell me what system they ...
Harriet Feilotter
GENE-ARRAYS@...
Jan 4, 2002 3:22 am
6327
We had the same problem with a ScanArray 4000 that we are using here. I also went through a long time trying to figure out whether it was slide or scanner...
Harriet Feilotter
GENE-ARRAYS@...
Jan 4, 2002 3:26 am
6328
Alexa Fluor 546 and Alexa Fluor 647 dyes also work well for microarray sample labeling. The publication "Tools for Microarray Analysis" lists the dUTP forms as...
Jill Hendrickson
GENE-ARRAYS@...
Jan 4, 2002 3:30 am
6329
Sir/Madam: I am interested in searching for drought-responsive genes in Brassica spp. May I request you to please guide me on how to proceed. Regards KC Bansal...
Kailash C. Bansal
GENE-ARRAYS@...
Jan 4, 2002 3:31 am
6330
01/03/02 Hello Rork, thank you for the interesting contribution. Your first note was very brief, so it was not apparent that the main concern of...
Piali, A ".gel" file is a 16 bit, square root encoded TIFF image. The file and header are fully compliant with TIFF standards and correctly recognized by many...
David Hanzel
GENE-ARRAYS@...
Jan 4, 2002 3:38 am
6332
Post-Doctoral Position (Research Officer, RO1-3) St. Vincent\'s Institute of Medical Research, Melbourne, Australia. Project area: Vascular Complications of...
Mark Waltham
GENE-ARRAYS@...
Jan 4, 2002 3:42 am
6333
Hi when we decided to buy cy3- or cy5 dUTP i asked quotes to both NEN and Amersham and got a better price from NEN ! hope this helps. ... ...
Dear Willy, I can see from your Dec. 28 post to the microarray group at Yahoo that you are interested in other sources of CY-labeled nucleotides. As far as I ...
Fred Hilerio
GENE-ARRAYS@...
Jan 4, 2002 3:47 am
6335
Dear Server, please use my new Address, Sirak...
Sirak Kifle
Kifle@...
Jan 4, 2002 11:03 am
6336
Hi, Has anyone ever used the aminoallyl labelling method with cRNA on Affymetrix GeneChips? If so, is there a protocol anywhere? Thanks, Matt....
mattboy73
mattboy73@...
Jan 4, 2002 12:18 pm
6337
Hi Folks, Can anyone give me some idea as to how many molecules of oligo and or PCR product tend to be spotted per 'standard' spot., 10E2, 10E4, 10E6 etc? Can...
Has anyone yet tried the small sample preparation technical note recently added on the Affymetrix website? It consists of a first round amplification using...
Has anyone done a side-by-side comparision of the formamide based hyb buffers and the SSC-based buffers? Assuming that using a 50% formamide/10%dextran/1%SDS...
Has anyone done a side-by-side comparision of the formamide based hyb buffers and the SSC-based buffers? Assuming that using a 50% formamide/10%dextran/1%SDS...
Dear friends We are trying to analyze microarray experiment results of human cDNA clone chips. We used four cDNA chips(include replication); Dye1 set (2 slide)...
Hoping that you will be able to attend my party next month. So far, we have 13 men, 10 women, and 16 couples that are comming. This should be the best ...
Dear friends I have a queation about the correlation for Cy3 vs Cy5 intensity. I knew that the there is the linearity relationship between cy3 and cy5. I...
Hi, when I use BLAST program, begining with a mus musculus' clone and, I only obtain Human sequences?...
genexpression2002
rmardone@...
Jan 7, 2002 3:55 pm
6346
Hi, Has anyone happened to know any vendors of spotted cDNA arrays containing >8000 human genes? Especially include neuroscience related ones? What is the...
hello everybody, I cant obtain the sequence for some MDB in the Gen Bank, if you know how I can obtain this information, please reply. Rene....
genexpression2002
genexpression2002@...
Jan 7, 2002 6:47 pm
6348
I was wondering how others normalize their data to cDNA quantity and flurochrome incorporation efficiency in dual-label microarray experiments. I was...
I am testing different surface chemistries on standard 1 x 3 x 1 glass microscope slides in order to find a more stable substrate for spotting. Does anyone...
raycalzada@...
Jan 8, 2002 12:19 am
6350
I am testing different surface chemistries on standard 1 x 3 x 1 glass microscope slides in order to find a more stable substrate for spotting. Does anyone...
TRANSCRIPTOME 2002: From Functional Genomics to Systems Biology, to be held in Seattle, Washington, USA, from March 10-13, 2002, is the latest in a series of...