Hi, I've been doing a number of hybs to mouse cDNA microarrays - the protocol I've been following involves a pre-incubation of direct labelled cDNA with human...
We have three Agilent 2100s. For the most part quantification of RNA works well. However, any quantities (10 ng or less per microliter) they aren't accurate....
rbchadwick
rbchadwick@...
Feb 1, 2002 4:16 pm
6638
Hi Robin, Try the following: 1. Web primer, http://genome-www2.stanford.edu/cgi-bin/SGD/web-primer 2. Primer selection, http://alces.med.umn.edu/websub.html ...
Hi, Mark and Steve, SwissProt can also be used to query for information on if a gene is expressed in a particular tissue. One potential pitfall in query dbEST...
Hao Chen
hchen@...
Feb 2, 2002 3:04 am
6640
I have recently entered the microarray "game". In doing so I purchased kits from multiple vendors and have tried the home method from the UCSF web site. I...
Nancy Shipley
GENE-ARRAYS@...
Feb 2, 2002 3:07 am
6641
I haven't had much problem with pricing on the Corning slides, but I have also been looking around. Initial tests on the FMB slides from Turner Design are...
Robert Searles
GENE-ARRAYS@...
Feb 2, 2002 3:09 am
6642
We are using the PAXgene RNA extraction system from Preanalytix to get RNA from total blood. We are having big problems since the yield does not seem to be as...
Elisa Jimenez
GENE-ARRAYS@...
Feb 2, 2002 3:11 am
6643
The average efficiency may be hard to measure, but as the conjugation is done in vast excess of dye, I would expect all the amino-allyl groups to conjugate. ...
Chris Seidel
GENE-ARRAYS@...
Feb 2, 2002 3:15 am
6644
We are working with neurons primary culture, which means very limited starting material and no way to rich the confluence. So, available amount of total RNA is...
Tetyana Pedchenko
GENE-ARRAYS@...
Feb 2, 2002 3:16 am
6645
Hello Everyone, A number of you have written me regarding some cases of Spam, specifically along what some would call a salacious line of thought, and I would...
Chandi Griffin
aniya@...
Feb 2, 2002 3:18 am
6646
Bart, my $0.02: The alcohol displaces water from macromolecules, making them more likely to interact with similar macromolecules. The sodium ion works to...
Rudolph Spangler
spanglr@...
Feb 2, 2002 3:25 am
6647
Dear Greg, it would be very nice to have these posters in PDF-format! Thanks a lot and best regards Doris ... Dr. rer. nat. Doris Steinemann Institut für...
Mailing List.
GENE-ARRAYS@...
Feb 2, 2002 3:27 am
6648
Steve, If you are finding the price of Corning slides in the UK too expensive, have you tried the equivalent SuperAmine slides from Telechem. They are ...
Mark Truesdale
GENE-ARRAYS@...
Feb 2, 2002 3:28 am
6649
Does anyone have any experience with all the different RNA labelling kits for microarrays - with regards to oligonucleotide arrays on glass slides. I am aware...
Marie Craigon
GENE-ARRAYS@...
Feb 2, 2002 3:30 am
6650
Elisa, Qiagen developed this product. If it's not working for you, I'm sure the product manager at Quiagen will guide you. I looked at the patent on this...
Rudolph Spangler
spanglr@...
Feb 2, 2002 3:32 am
6651
Lev, We at Molecular Probes have been able to label amine-modified DNA with most dyes with efficiencies up to about 1 dye per 8 bases. However, this labeling...
Vicki Singer
GENE-ARRAYS@...
Feb 2, 2002 3:33 am
6652
Dave We don' recommend or do it, and I don't know of any protocols for this You would need to keep arrays wet and then do high temp/low salt washing. On...
Douglas Roy
GENE-ARRAYS@...
Feb 2, 2002 3:35 am
6658
Hello- Regarding the use of the "COMMERCIAL" tag on post: The "COMMERCIAL" tag MUST be the first term in the subject line of ALL commercial post. This policy...
Chandi Griffin
aniya@...
Feb 2, 2002 4:04 am
6659
Hi, At our lab we have wash problems after hybridisations. Normally we wash in 0.5x SSC and twice in 0.2x SSC. We wash the slides in Flacontubes and we use a...
ALMA Bioinformatica SL (http://www.almabioinfo.com) is proud to announce that its propietary solution for expression data analysis, SOTA, is available both for...
With regards to keeping dyes anhydrous, is it sufficient to use silylation grade reagents (stored in a dessicated container), and store the reconstituted dyes...
Hello, I have done a number of reciprocal labelling experiments using the direct cy3/5-dUTP method. A majority of genes showed no differential expression but...
Dear Tetyana, We have had experience using Arcturus amplification kit http://www.arctur.com/products/riboamp_rna_amplification.htm . We were able to isolate...
Gregory Khitrov
khitrog@...
Feb 5, 2002 3:12 am
6666
Dear Vannessa, I read your problem that you have send to the microarray group about black holes. We have the same problem in our lab. I also read the reply's...
Dear all, I would be grateful for a few references where partial (i.e. not targeting the whole genome, only a smaller part of it) oligonucleotide microarrays ...
Jolin, Lisa would like to recall the message, "[microarra-group] Digest Number 465". ********************************************************************** ...
Dear all, I'm working with chips spotted with small numbers of genes (usually 50-100). Using so few genes, I understand that it's preferable to normalize your...
Dear members, I have several question, what is the main concept of the datamining in array data? I know several methods such as clustering, k- means, principle...
