Hello everyone, In our lab, we are interested in doing ourself the slide coating. Is there any method of making traitment without immersion of the slide in any...
David, I have not used casein, but can recommend bovine serum albumin (BSA; Life Technologies sells a high quality molbio grade, cat # 155610-12) or chicken...
Canel, Camilo
canelc@...
Mar 5, 2002 8:59 pm
6730
I agree that it is essential to generate information about variability among biological samples. As some have pointed out, pooling the experimental samples...
Canel, Camilo
canelc@...
Mar 5, 2002 9:16 pm
6731
Dear Microarray Group We (scGTI) have circulated the information below in a variety of ways already, but there may be a lot of members in our microarray group ...
COMMERCIAL POST: Emerging Protein Microarray Application Dear Microarray Discussion Board Participant, In 2001, Prolinx released its Versalinx Protein...
Scott, ... It's slightly difficult to comment on this without having all the details. However I suggest to look at this tool which might give you some ...
jacobsen
jacobsen@...
Mar 6, 2002 10:07 pm
6734
Any sources for an Affymetrix U95Av2 CDF file? Other Affy CDFs sought as well. Thanks! BRET...
Karoly, Your point is very well taken. In the case of the pyramidal cells you are studying, you may need to collect more samples to detect statistically...
Canel, Camilo
canelc@...
Mar 7, 2002 3:13 am
6736
All, I am a neurobiologist and have quantitated mRNAs for over 20 years by In Situ in rodent, primate and human brain. Of the several hundred mRNAs we have...
Watson, Stanley
watsons@...
Mar 7, 2002 3:23 am
6737
Dear George, we purchase our Mouse and Human ready to print libraries from Research Genetics and they seem to be very good. Price wise its reasonable. Hope...
Gregory Khitrov
khitrog@...
Mar 7, 2002 3:26 am
6738
... No, I am not implying that at all. Users must decide what statistical tests to apply based on the experimental design, their knowledge of the system, and...
Canel, Camilo
canelc@...
Mar 7, 2002 3:27 am
6739
For tough tissue, take the entire tissue (100 mg) and put it in triazol (1 ml) with ~ten 3 mm Zirconium beads and homogenize in a 2 ml screw top tube for 1...
We are looking for 384-well, v-bottom, polystyrene plates for printing small volumes of oligonucleotides. Can anyone suggest a source? Thanks, James Fuscoe,...
Dear students, CLONDIAG offers a 4 to 6 month internship for a diploma student of a technical college or university where you are given the opportunity to work...
Bioslide Technologies of Walnut, California introduces the Precision CT®-Amine, Precision CT®-Clean and Precision CT®-Aldehyde slides. Bioslide Technologies...
Peter, Needless to say, you should strive to rid your RNA samples of any trace of genomic DNA from the source cells. I don't recommend DNAse treatment because,...
Canel, Camilo
canelc@...
Mar 8, 2002 5:05 am
6745
Some time ago, I tried to explain the preprocessing steps in micro array analysis to a group of colleagues. There was confusion, because "normalization"...
Nick Tinker
tinkerna@...
Mar 8, 2002 5:24 am
6746
I would like to direct members to a useful web-based program for analyzing Affymetrix DNA microarray data called ArrayExpress at...
Dear all, I am interested in the automated hybridization systems. I tried Ventana, GeneTac and the one from Amersham. I obtained the best results with...
Hello, Many of you have probably struggled with the notorious problem of deciding *a priori* the number of clusters a non-hierarchical clustering method ...
Hi: Thank you all for your responses to my previous query regarding the PCR purification protocol. I have a few spotting related questions need to be...
Hi folks, I find the Sequence Extract program very useful for locating the binding sites of oligos to their template sequences. This free program was written...
Rudy, Thanks for the kind words. To replicate our array data we tend to first replicate our own chip data, often across a different set of external...
Watson, Stanley
watsons@...
Mar 15, 2002 3:06 am
6752
Rudy, Statisticians have repeatedly said to me that they will have a methodology worked out for properly analyzing microarray data. I am still waiting. I...
Canel, Camilo
canelc@...
Mar 15, 2002 3:23 am
6753
Vesarat, If I understand you correctly, you have attempted to apply ANOVA to groups of genes in which only a small subset are differentially expressed. I am...
Canel, Camilo
canelc@...
Mar 15, 2002 3:25 am
6754
... I would like to know if any one has been studying cytokine expression with cytokine micro-arrays and have seen meaningful results? Thanks in advance Neil ...
Hi Azadeh Badiee, ... If you speak about quality assessment and standardisation you should also speak about the data annotation and management. Most...
Hi all, I'm writing my PhD thesis about the statistical analysis of microarrays. Is there a good book about e.g. what is a microarray, why is it used, what is...
