Hi, I am a assistant research scientist involved in setting up and validating microarray technology in our company.I have been using SYBR green II to stain ...
Hi Walter, Try SpotBot from Arrayit Telechem. http://arrayit.com/Products/Printing/SpotBot_Complete/spotbot_complete.html E ... From: Walter Schick To:...
Hi Tina, Are you running purified PCR products? In this case you may have multiple bands because of partial denaturation of your PCR products due to the ...
Does anybody have any experience with microarrays being used for follow up of the clinical trials? Especially experiences with blood RNA expression profiling...
Hello: I am not suggesting that the cross-species hybridization should be done, but I am looking if anybody has already done this!! More specific, I am looking...
Hello all! My lab studies DNA replication in S.cerevisiae and I have been working to apply array technology to our research needs. I am using synchronized 1N...
Using arrays in clinical trails is tricky. We just started doing some skin-related clinical trails, and have conducted some array studies. Would be glad to...
Hi there, I am printing 60mers oligos on poly-lysine coated slides using 10% DMSo, 3X SSc as my printing buffer. Can someone advise what is the usual...
Hi, I am thinking of purchasing an Omnigrid arrayer and a set of pins. I would welcome any comments on the Omnigrid arrayer, its reliability, ease of use,...
Do you need to use the midi or maxi RNeasy kits for that much blood? Also, do you lyse the RBCs and do you find it to be complete? Hank Wolfe ... From: "Vine,...
Hello, I was wondering if the amount of cDNA hybridized in one spot is linear correlated with the expression value. Could somebody help me, please? Thanks a...
Does anyone have a recommendation for a vendor selling a comprehensive human cDNA microarray (or set of arrays)? I would like something that covers U133 or...
"Microarray Data Mining in Xpression NTI" workshop will he held at the Experimental Biology 2002 Conference on April 20-24th, 2002 from 3:30pm - 4:30pm in New...
Hi, Is there any one test what is the best concentration of formamide to use for cDNA array hybridization: 50% or 25% of final concentration? Does it give...
Does anyone know of a commercial reference RNA that would be suitable to use in a comparison of reference types with the goal of differentiating between...
Hallo everyone, another question: What do you think about an alternative to RT and direkt/indirekt labeling: The efficiency is quite poor.(My experience using...
Dear everyone, has anyone experience in extracting RNA from paraffin-embedded material? Which method do you use, what is the amount and quality of total RNA or...
I have developed a unique database architecture that permits comprehensive indexing of the functional attributes of proteins. If you are interested in...
I suggest you to rince your slides very briefly (15 seconds) in water as a last wash. It works fine for me. Hope that helps Stephan Soullier Centre of...
Dear members: My project involves studying gene expression in clinical isolates of yeast (studying gene expression ex-vivo).I am working with clinical samples...
Hi Vera, I am using 17-26 mer oligos and labelled RNA as target (I am developing microbial diagnostic DNA arrays based on 16S rRNA sequences). The difference ...
We are working with a Genemachines spotter and an Axon scanner and are satisfied with both of them. From earlier posts to this group it appears that the...
This came from the QPCR discussion group, if you are a registered Yahoo user: Yes, Yahoo is trying to make some additional income, so they have automatically...
I have couple of questions and any comment or answer is appreciated: 1. How the replicates treated , do we take average? if one spot had totally different...
I can try to answer the last question: First: normalization should be defined - it can have three different statistically valid points: 1) Standardization - a...
Lucy, Another approach would be to plot the replicates vs. each other on log-log axes - the straighter and thinner the "cloud", the better. Chris Hawkes, Ph.D....
Hello List Members, I am wondering about species discrimination using microarrays! Could some one please share the strategy used to design the species specific...
Dear Mehrdad, I have not worked with time points however I know some web-pages, where you might find some interesting articles. I understand, that you think...
