Dear Elsebrit, Thank you for using our slides. Corning GAPS II slides offer a very flat surface for printing microarrays. We have had no problems like the one...
Canel, Camilo
canelc@...
Jun 5, 2002 3:03 am
6928
Hi all: I am a chip newbie and am looking for a file(if already generated) that can link Affy probe numbers and gene function(Ontology). I have been asked to...
Dear Sir/Madam, We are conducting a survey on microarray glass slide usage to accurately interpret the product and marketing trends. This survey will aid in...
Dear everybody: I am a teacher at a university and wonder which kind of public software and database appropriate for the classes devoted for array (gene...
Just a reminder, the third annual Northwest Microarray Conference is right around the corner. We have a few presentation slots still available, a few vendor...
... I believe GenBank has a batch query facility. Alternatively, you can download up to 10,000 results into a single file. Depending on the species you're...
Lucy McWilliam
lejm3@...
Jun 13, 2002 12:20 am
6934
Dear Kim if you still want to order sequence verified human cDNA we have purchased ready to print from ResGen. The ready to print library is very good. We...
Greg Khitrov
khitrog@...
Jun 13, 2002 2:05 am
6935
You could try the Godlist Manager I wrote, except you would not get the chromosome location. The software can be downloaded from...
Hello! I was hoping to get suggestions on the best type of 384 well plates to use for printing. In particular, I was hoping to get opinions on using plates...
Holly Preston
hpreston@...
Jun 13, 2002 10:15 pm
6937
Hi Holly- Unfortunately I have little opinion on a round bottomed well plate. The type of plate you will want to use will also depend on your procedure, if...
Thanks everyone for all the interesting discussions and valuable inputs. I feel very fortunate to be a member of this group for the past 16 months.Goodluck ...
I store my Cy3 labelled RNA for months without significant signal loss. However this RNA is generated via IVT from PCR products. Moreover, with Cy5 it is quite...
I'm not sure whether this has been addressed here, but has anyone had any experience in storing their fluorescent cDNA for a few weeks/months prior to...
The oldest reference for linear polyacrylamide as a carrier for ethanol precipitation, that I know of, is: Gaillard, C. and Strauss, F. (1990) Nucl. Acids,...
Hi All, Please let me know if there is anybody familiar with usage of multiclass response choice in SAM Analysis. I need to use this to compare expression...
Well, I'm one of the big fans of RNAlater and have stored tissues in RNAlater at -80 indefinitely. Because it precipitates the RNases, they are effectively...
Hi All- I've been using a set of 4854 rat oligos purchased from Compugen (and made by Sigma-Genosys). These are all 65-nt, 5'-aminated oligos with very...
Has anyone used the picogreen kit from molecular probes for quantitation of DNA in presence of RNA? Can I use it in place of a ribosomal gel/Agilent 2100 to...
I'm using amersham post-labelling kit and I have some problems with a high background in the Cy5 channel (the the Cy3 is perfect!). Does anyone have any...
We've been using RNAlater to transport exotic tissues with excellent results. For example, the Chinese crab Eriocheir sinensis is a prohibited species in the...
I use commercially available reference RNA (mixture of cell lines) for all the experiments. My experimental sample is from tissue and I have different time...
A customer has been bring Candida albicans total RNA samples to our core facility for analysis on the Agilent Bioanalyzer. They have isolate RNA by a hot ...
Dear Jean-Baptiste, Corning UltraGAPS slides offer an excellent substrate for printing oligonucleotide arrays of the size that you want to use. Please visit...
Canel, Camilo
canelc@...
Jul 4, 2002 3:08 am
6956
The standard of validation of microarray slides is fairly poor. When you buy a slide you really have no idea if every spot is present and whether it will...
