hello again Mr maider, i found the problem in fact i must begin with i=2:n and replace i+1 per l=i+1 for example. but the loop has taken 20 to 30 minutes, so i...
Hi, everyone, We have built a multiplex SNPs genotyping system by using oligonucleoide microarray. Now, we are looking for good software which can be easily...
lmjcxj
lmjcxj@...
Apr 1, 2003 5:35 pm
9481
BioFiber Identification, Inc. develops life science research and medical diagnostics products based on proprietary technologies in the areas of proteomics and...
Hi Elena! I have had great success reducing background with a prehyb. I use 4X SSC, 0.1% SDS, 0.2% SSC fro 20 minutes at 50 degrees C. Use the prehybed...
Joseph Andrews
GENE-ARRAYS@...
Apr 2, 2003 3:07 am
9483
Hi, I have a question regarding normalising a 2 colour array. In the experiment I'm working on the vast majority of genes in the control sample will, in time...
Anthony Curran
GENE-ARRAYS@...
Apr 2, 2003 3:16 am
9484
Dear Gene-Array List Server readers, Technical issue on the subject of spotting PCR DNA. According to the report of Diehl., et al 2002 (Nucleic Acids Res 2002...
Duval, Manuel
GENE-ARRAYS@...
Apr 2, 2003 3:16 am
9485
Hi, What you can tell me about the use of liquid nitrogen to homogenize tumor samples to extract RNA? The froozen sample is put in a porcelain crucible and...
silviacrusco
silviacrusco@...
Apr 2, 2003 3:17 am
9486
www.Metrigenix.com Mo Hashemzadeh Director, Business Development MetriGenix, Inc. 708 Quince Orchard Road Gaithersburg, MD 20878 Tel: 301-527-2235 Fax:...
Mo Hashemzadeh
mhashemz@...
Apr 2, 2003 3:18 am
9487
... From: Boles, Chris Sent: Wednesday, March 26, 2003 1:50 PM To: 'To share info regarding GeneChips tech. and Microarrays in general' Subject: RE: background...
Boles, Chris
GENE-ARRAYS@...
Apr 2, 2003 3:18 am
9488
Hello, Dose anybody has Moleculer Dynamics GenIII type printer for sale? We are looking for used Moleculer Dynamics GenIII printer. If somebody has this...
Hi, I think you should be careful throwing away genes or excluding hybridization events from normalization. You do not want to remove spots from normalization ...
peter morrison
petermo@...
Apr 2, 2003 3:20 am
9490
... From: Boles, Chris Sent: Tuesday, March 25, 2003 8:50 AM To: 'To share info regarding GeneChips tech. and Microarrays in general' Subject: RE:...
Boles, Chris
GENE-ARRAYS@...
Apr 2, 2003 3:21 am
9491
Hi Let first clarify the issue of ontologies, molecular function and at the end it add some additional comments about clustering GO Under the Gene Ontology...
Willy Valdivia Granda
GENE-ARRAYS@...
Apr 2, 2003 3:22 am
9492
Try going to biocompare website , you will find very good information there which you are looking for. Regards Vishal ... From: Miroslava Cuperlovic-Culf...
VISHAL GANDHI
vishal.gandhi@...
Apr 2, 2003 4:15 am
9493
I will be out of the office starting 04/01/2003 and will not return until 04/03/2003. I'm sick. Hopefully in tommorrow. -Dave...
just looking for positive feedback on any automated hyb stations anyone has used. wanting to purchase the instrument that would best fit our application. Rich...
A few questions for the "seasoned" arrayers. I am using a GeneTAC hybstation. I am having a bit of trouble with two things. First, in trying to optimize the...
zimmerman1microarray
zimmerman1@...
Apr 2, 2003 8:23 pm
9496
Ok, I'm 18 and a freshman in college! Just got my first Webcam and want to Fuck in front of it for you! If your interested and Serious then drop me a line! ...
Hi all, I've been using ethanol precipitation to remove unincorporated Cy3(5) dyes. However, after made several controls I realized that free dye precipitates ...
Ulises Urzua
GENE-ARRAYS@...
Apr 3, 2003 3:03 am
9499
GeneSpring 5 has an amazing flexibility for all of the possible normalization schemes, including the capability of selecting subsets of genes for...
Hi All- We have a Flexys Robot, but it does not have a humidity-control system. I am wondering if anyone knows a portable system that I can put in the hood to...
Ji Qiu
GENE-ARRAYS@...
Apr 3, 2003 3:13 am
9501
If you have a high confidence level that your control genes will have a relatively consistent expression level on all the slides, my opinion is that using...
Holton, Dile
GENE-ARRAYS@...
Apr 3, 2003 3:14 am
9502
Dear Colleagues Most protocols require the lyophilisation of direc, or indirectly dye labelled probes. We have usually used a speedvac for this. However, we...
Samuel Breit
GENE-ARRAYS@...
Apr 3, 2003 3:14 am
9503
Anthony, Here is a paper describing how we solved this problem: http://www.nature.com/cgi-taf/DynaPage.taf?file=/embor/journal/v4/n4/abs/emb or798.html If you...
Frank Holstege
f.c.p.holstege@...
Apr 3, 2003 4:13 am
9504
Hi guys, I just joined the group and wanted to give you all my site to check out. You should like it, no matter what you are in to: ...
Does anybody know the nearest place to Memphis, TN where I might get training in the following ? I was looking at an article ("Fingerprinting Vegetables"...
Dear Affymetrix GeneChip users, We are planning to rehybridize our old hyb cocktails to the new generation of chips. We have only 110 ul left of hyb cocktail...